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To maintain anesthetic depth, half-doses were administered every 90 minutes, with adequacy confirmed by the absence of withdrawal and auricular reflexes, as previously described in experimental animal models.31,72,73 The abdomen was shaved, disinfected with 10% povidone- iodine (Batticon, Adeka, Turkey), and a midline laparotomy was performed

Exposure to heavy metals such as mercury, cadmium, lead, or arsenic can also lead to glutathione depletion

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